Making TLC Plates coming from Bulk TLC Silica Skin gels
Many TLC users choose to use pre-coated TLC discs, but other folks because of their exceptional needs, or perhaps because they require special additives or a particular thickness, coating their own TLC plates. This paper is a quick review of what is needed for the process.
Primary instructions intended for plate covering:
If reproducible plates will be needed, then a user ought to invest in a coating device from Camag or perhaps Desaga (addresses below). The devices consist of a holder that holds the goblet plates, and a device maintain slurry to become placed on the glass china. A guide enables the applicator device (with an adjustable layer thickness gauge) being pulled over the glass dishes as the slurry can be dispensed. Several practice is needed to get a constant body movement pulling the applicator over the plates to get a level layer. The glass dishes must be similar thickness and extremely clean. Any oil or perhaps grease will result in a poor layer. Handle the plates with rubber hand protection when cleansing or rinsing. Care is additionally needed in case the edges of these plates are not polished or finished off, since a serious cut might result. Only if coating a few plates, then the glass platter can be piled up on two opposite edges with masking tape to the appropriate elevation to give the width needed. Slurry is added on to one particular end from the plate and a goblet rod that extends beyond the masking taped edges may be used to pull the slurry equally over the menu. There will be considerable waste before the correct amount of slurry for the thickness essential is worked out from learning from your errors. Smaller TLC plates may be coated by using smaller cup plates (microscope slides) and placing a pair of them continual. The two photo slides are dropped into the silica (or other) slurry, picked up, separated and dried. If the small beaker is used and methanol instead of water is employed for the slurry, then a silica gel slurry can be saved and used again.
Binders and TLC:
It is important that anyone making...
References: 1) E. Stahl, Thin Part Chromatography, Springer-Verlag, New York, 1969 2) Camag Scientific, Inc., 515 Cornelius Harnett Drive, Wilmington, NC 28401, mobile phone 800-3343909, send 919-343-1834 3) Desaga GmbH, P. Um. Box 101969, D-69009 Heidelberg, Germany, telephone 0-62-21-83590, send 0-62-21-840887